IEEE - Institute of Electrical and Electronics Engineers, Inc. - Expression and analysis of Mycobacterium bovis mpb63–64 fusion gene in Escherichia coli

2010 3rd International Conference on Biomedical Engineering and Informatics (BMEI)

Author(s): Fei Ren ; Chunfang Wang ; Lulu Li ; Xinyu Liu ; Xiuyun Jiang ; Lei Liu ; Bingjie Li ; Haoran Ning
Publisher: IEEE - Institute of Electrical and Electronics Engineers, Inc.
Publication Date: 1 October 2010
Conference Location: Yantai, China, China
Conference Date: 16 October 2010
Volume: 5
Page(s): 2,041 - 2,044
ISBN (CD): 978-1-4244-6497-5
ISBN (Electronic): 978-1-4244-6498-2
ISBN (Paper): 978-1-4244-6495-1
DOI: 10.1109/BMEI.2010.5639495
Regular:

The DNA fragments of mpb63 and mpb64 were fused by splicing by overlapping extension(SOE) polymerase chain reaction(PCR), and the fusion gene mpb63-64 was cloned into pMD18-T vector, then we got... View More

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